Fig. 7: Trim26 deficiency in hepatocyte promotes hepatocyte proliferation through Wnt/β-catenin signaling pathway. | Cell Death & Disease

Fig. 7: Trim26 deficiency in hepatocyte promotes hepatocyte proliferation through Wnt/β-catenin signaling pathway.

From: TRIM26 deficiency enhancing liver regeneration through macrophage polarization and β-catenin pathway activation

Fig. 7

A Primary hepatocytes were cultured in complete medium for 24 h, then the relative expression of cell cycle proteins was detected. B–E Representative graphs of flow cytometry analysis of L02 cell cycle, and the statistical analysis of percentages of cells at different cell cycle stages (G0/G1, S and G2/M). F L02 cells were transfected with Trim26 shRNA, and cell lysates were subjected to WB. G L02 cells were transfected with Trim26, and cell lysates were subjected to WB. H L02 cells were transfected with Trim26 shRNA. After treatment with or without ICG-001 (10 µM/mL) for 24 h, cell lysates were subjected to WB. I L02 cells were transfected with FLAG- Trim26. After treatment with or without MG132 (10 µM/mL) for 6 h, cell lysates were subjected to WB. J Representative immunoblotting bands showing the indicated proteins in the shTrim26 and shControl L02 cells transfected with HA-tagged ubiquitin, Flag-β-catenin, and MG132 treatment. (*p < 0.05, **p < 0.01, ***p < 0.001).

Back to article page