Fig. 5: GSDME complexes with PALS2.
From: Genetic influence on vascular smooth muscle cell apoptosis
A, B Lysates of vascular smooth muscle cells were subjected to immunoprecipitation (IP) with the use of either an anti-GSDME antibody (Thermo Fisher, MA5-36092) (lane 2 in A), an anti-PALS2 antibody (Thermo Fisher, PA5-21889) (lane 2 in B), or an IgG isotype control antibody (Thermo Fisher, 10500 C) (lane 3 in A, B), with cross-linking of antibodies to Dynabeads to reduce the IgG heavy chain band, followed by Western blotting with the anti-GSDME antibody or the anti-PALS2 antibody, as indicated in the figure. C, D Membrane fraction of vascular smooth muscle cells were subjected to IP with the use of either the anti-GSDME antibody (lane 2 in C), the anti-PALS2 antibody (lane 2 in D), or the IgG isotype control antibody (lane 3 in C, D), followed by WB with the anti-GSDME antibody or the anti-PALS2 antibody, as indicated in the figure. IgG-HC IgG heavy chain. A–D Lane 1:5% of the amount of the protein extract used for immunoprecipitation. M: protein size markers.
