Fig. 4: Structures of VHH60 binding to SARS-CoV-2 spike protein.
From: A broad neutralizing nanobody against SARS-CoV-2 engineered from an approved drug
a Cryo-EM for the Spike-VHH60 complex observed to a resolution of 3.79 Å, revealing binding of VHH60 to RBDs in the “1-up” conformation. b Comparison of the epitope between VHH60 (light sea green) and VHH72 (salmon, PDB ID: 6WAQ). c CDR loops of VHH60 (cornflower) overlaid on the surface epitope (salmon) representation of RBD (light sea green). d The hydrogen bonds at the binding interface between VHH60 and SARS-CoV-2 RBD based on the crystal structure of the complex at 3.40 Å. e Superposition of the RBD-ACE2 model (PDB ID:7XO9) to that of the VHH60- RBD model shows there is a steric hindrance between VHH60 and ACE2.
