Fig. 5: YME1L overexpression induces pro-tumorigenic activity in primary human NPC cells.
From: Expression and functional implications of YME1L in nasopharyngeal carcinoma
The stable pNPC-1 primary NPC cells with the lentiviral YME1L-expressing construct (oeYME1L-slc1 and oeYME1L-slc2, two stable selections) or the vector (“vec”) were established and expression YME1L (mRNA and protein) was examined (A and B). The exact same number of the above pNPC-1 cells were further cultivated for designated hours, the mitochondrial complex-1 activity (C) and ATP contents (D) were tested; Cell proliferation, viability, migration and invasion were tested via nuclear EdU fluorescence staining (E), CCK-8 (F), “Transwell” (G), and “Matrigel Transwell” (H) assays, respectively. Primary NPC cells that were derived from other patients, pNPC-2/-3/-4, with the lentiviral YME1L-expressing construct (“oeYME1L”) or the vector (“vec”) were formed and expression of YME1L mRNA was tested (I); ATP contents (J), cell proliferation (K) and migration (L) were tested similarly. The numerical values were mean ± standard deviation (SD, n = 5). *P < 0.05 vs. “vec” cells. Experiments in this figure were repeated five times, and each time similar results obtained. Scale bar = 100 μm.
