Fig. 6: YME1L is important for Akt-mTOR activation in NPC cells.
From: Expression and functional implications of YME1L in nasopharyngeal carcinoma
The stable pNPC-1 primary NPC cells with the lentiviral YME1L shRNA (“kdYME1L”), the lentiviral CRISPR-YME1L-KO construct (koYME1L), or the lentiviral scramble control shRNA plus the lentiviral CRISPR-KO control treatment (“ctrl”), the lentiviral YME1L-expressing construct (oeYME1L-slc1 and oeYME1L-slc2, two stable selections) or the vector (“vec”) were cultivated for 12 h, expression of listed proteins was tested (A and B). kdYME1L pNPC-1 cells were further stably transduced with a S473D constitutively-active mutant Akt1 (caAkt1) or the empty vector (“Vec”), expression of listed proteins was shown (C). These cells were further cultivated for designated hours, cell proliferation, migration and apoptosis were examined via EdU-nuclei staining (D), “Transwell” (E) and TUNEL-nuclei (F) assays, respectively. The numerical values were mean ± standard deviation (SD, n = 5). * P < 0.05 vs. “pare”/“vec” cells (A and B). # P < 0.05 (C–F). “N. S.” stands for non-statistical difference (P > 0.05).Experiments in this figure were repeated five times, and each time similar results obtained. Scale bar = 100 μm.
