Fig. 2: Differential phosphorylation between wild-type and mutant tau. | Cell Death & Disease

Fig. 2: Differential phosphorylation between wild-type and mutant tau.

From: TauP301L disengages from the proteosome core complex and neurogranin coincident with enhanced neuronal network excitability

Fig. 2

A Immunoblot of eGFP-Tau that has been immunoprecipitated by GFP-Trap® and probed with antibodies for different phospho-tau epitopes – AT8 (pSer202, Thr205), pSer262 and pSer396 – as well as GFP, which was used as an estimation of total human tau levels. B The levels of eGFP-TauP301L phosphorylation relative to eGFP-TauWT. The dotted line represents an equal amount of the phosphorylation site between both systems. Significant increase in phosphorylation at Ser262 but not AT8 or Ser396 with eGFP-TauP301L (ratio-paired t-test of raw data, n = 3, pSer262, *P < 0.05; AT8, P = 0.089; pSer396, P = 0.549). Error bars = ±SD. Immunofluorescence of transduced DIV13 neurons probed for AT8 (C) or Ser262 (D) (red). Images taken with the EVOS M7000 imaging system. Scale bar = 100 μm.

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