Fig. 3: Workflow for determining the tau interactome by AP-MS. | Cell Death & Disease

Fig. 3: Workflow for determining the tau interactome by AP-MS.

From: TauP301L disengages from the proteosome core complex and neurogranin coincident with enhanced neuronal network excitability

Fig. 3

A DIV13 primary neurons, transduced with AAV’s to express eGFP, eGFP-TauWT or eGFP-TauP301L were lysed in 1% Triton-X-100 lysis buffer and the cell lysates were immunoprecipitated with GFP-Trap® magnetic beads. The samples were digested on the beads and then labelled with TMT reagents and pooled before analysis by LC–MS/MS. Figure created with BioRender.com.

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