Fig. 4: The tau^WT interactome in primary cortical neurons.

A The STRING interaction network, incorporating both functional and physical protein associations, of 481 proteins identified as candidate tau interactors by AP–MS. Clusters were formed by MCL clustering (MCL inflation parameter = 1.8) of the interaction network. 11 clusters in total were identified, the five largest clusters are highlighted. Functional enrichment analysis of each cluster was undertaken for GO: Biological Process (BP), GO: Molecular Function (MF), GO: Cellular Compartment (CC) and KEGG. The most significant descriptor (assessed as the term with the smallest false discovery rate (FDR)) for each enrichment category is listed unless it was irrelevant to neurons, in which case the next most significant descriptor was included. The FDR for the top term in each cluster for each sub-ontology (GO:BP (B), GO:MF (C), GP:CC (D), KEGG (E)) is shown in order of significance, with cluster number in brackets next to the term, highlighting that eGFP-Tau^WT is most significantly enriched with proteins associated with the ribosome and translation (cluster 2). Both clustering and functional enrichment analysis were undertaken in STRING-db (v11.5). GO Gene Ontology, KEGG Kyoto encyclopaedia of genes and genomes.