Fig. 1: The higher expression of TRIM8 in HCC is related to poorer prognosis of HCC patients.

A The expression of TRIM8 in HCC tissues (T; n = 424) and adjacent non-tumor tissues (N; n = 50) in the TCGA database. ***P < 0.001. Welch t’ test. B Statistical analysis of TRIM8 expression in HCC tissues (T; n = 371) and adjacent non-tumor tissues (N; n = 50) in the TCGA database, and in normal liver tissues (N; n = 110) in GTEx database. ***P < 0.001. Welch t’ test. C, D Kaplan–Meier curve and the log-rank test were used to determine the diseases-free survival (DFS) and recurrence-free survival (RFS) in HCC patients with high or low TRIM8 expression based on TCGA database (http://www.kmplot.com/analysis/). E The ROC curve of TCGA-LIHC according to the expression of TRIM8. F RT-qPCR analyses of TRIM8 mRNA in paired tumor tissues (tumor) and adjacent non-tumor liver tissues (normal) from 85 HCC patients. *P < 0.05, Wilcoxon matched pairs test. G Semi-quantification of TRIM8 protein expression detected by Western blotting analysis in paired tumor tissues (tumor) and adjacent non-tumor liver tissues (normal) from 20 HCC patients. *P < 0.05, Wilcoxon matched pairs test. H Overall survival (OS) compared between 82 patients with HCC presenting low (n = 41) or high TRIM8 (n = 41) mRNA expression based on the cut-off value of the median value of TRIM8 using the log- rank test.