Fig. 4: The combinations of Syrosingopine and UK-5099 promoted mitochondrial damage and metabolic dysfunction by inducing lactate accumulation and oxidative stress. | Cell Death & Disease

Fig. 4: The combinations of Syrosingopine and UK-5099 promoted mitochondrial damage and metabolic dysfunction by inducing lactate accumulation and oxidative stress.

From: Syrosingopine and UK5099 synergistically suppress non-small cell lung cancer by activating the integrated stress response

Fig. 4

A Lactate secretion in the cell medium was measured after various treatments (n = 3). B–D The effects of syrosingopine, UK-5099, or their combination on ROS (B), MDA (C), and SOD (D) levels were assessed in H661 and PC-9 cells (n = 3). E Mitochondrial membrane potential was detected by JC-1 staining following various treatments (n = 3). F Intracellular ATP levels were detected using an ATP assay kit in H661 and PC-9 cells (n = 3). G Glycolysis in H661 and PC-9 cells treated with syrosingopine, UK-5099, or their combination was measured by assessing cytoplasmic acidification (n = 3). H Oxygen consumption in H661 and PC-9 cells treated with syrosingopine, UK-5099, or their combination was detected by oxygen consumption analysis (n = 3). (P-values: not significant (ns) p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

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