Fig. 5: The combination of Syrosingopine and UK-5099 triggers an integrated stress response through HRI activation. | Cell Death & Disease

Fig. 5: The combination of Syrosingopine and UK-5099 triggers an integrated stress response through HRI activation.

From: Syrosingopine and UK5099 synergistically suppress non-small cell lung cancer by activating the integrated stress response

Fig. 5

A RT-qPCR to confirm the expression of genes involved in ISR (n = 3). B The level of ATF4, P-eIF2α, and eIF2α in H661 and PC-9 after various treatments was analyzed by western blot (n = 3). C Analysis of cell cycle distribution in H661 and PC-9 cells treated with Syrosingopine and UK-5099 after knockdown of four eIF2α kinases (n = 3). D Western blot analysis of ATF4, P-eIF2α, and eIF2α levels in H661 and PC-9 cells treated with Syrosingopine and UK-5099 following knockdown of four eIF2α kinases (n = 3). (P-values: not significant (ns) p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

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