Fig. 6: The synergistic suppression effect of the combination of Syrosingopine and UK-5099 is dependent on the ISR. | Cell Death & Disease

Fig. 6: The synergistic suppression effect of the combination of Syrosingopine and UK-5099 is dependent on the ISR.

From: Syrosingopine and UK5099 synergistically suppress non-small cell lung cancer by activating the integrated stress response

Fig. 6

A Cell cycle analysis assessed the impact of ISRIB intervention on H661 and PC-9 cells (n = 3). B Western blot analysis was performed to determine the levels of ATF4, P-eIF2α, and eIF2α in H661 and PC-9 cells after ISRIB intervention and treatment with Syrosingopine and UK-5099 (n = 3). C Cell cycle analysis revealed that the mutation of Ser51 to Ala51 in eIF2α significantly inhibited the response to Syrosingopine and UK-5099 in H661 and PC-9 cells, compared to cells with wild-type eIF2α (n = 3). D Western blot analysis showed that he mutation of Ser51 to Ala51 in eIF2α significantly reduced ATF4 and P-eIF2α expression following treatment with Syrosingopine and UK-5099 in H661 and PC-9 cells (n = 3). (P-values: not significant (ns) p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

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