Fig. 2: Knockdown of MIF reduces LPS-induced mitochondria damage through activating the PINK1-Parkin pathway of mitophagy. | Cell Death & Disease

Fig. 2: Knockdown of MIF reduces LPS-induced mitochondria damage through activating the PINK1-Parkin pathway of mitophagy.

From: Macrophage migration inhibitory factor (MIF) suppresses mitophagy through disturbing the protein interaction of PINK1-Parkin in sepsis-associated acute kidney injury

Fig. 2

a The levels of MIF, caspase3 (Casp3), cleaved-Casp3, PINK1, Parkin, and GAPDH in the cytoplasmic fraction (Cyto), as well as PINK1, Parkin, VDAC1 in the mitochondrial enriched fraction (Mito), were assessed using immunoblotting. The relative expression of these proteins was represented as ratios: b PINK1/GAPDH, c Parkin/GAPDH, d cleaved-Casp3/GAPDH, e MIF/GAPDH, f PINK1/VDAC1, and g Parkin/VDAC1. h Representative images of JC-1 stained HK-2 cells were provided, with the green channel (monomer) and red channel (aggregate) merged, and a scale bar of 50 μm included. i The number of JC-1 stained HK-2 cells was quantified by FCM in both the green and red channels. j The depolarization of ΔΨm was evaluated based on the ratio of monomer/aggregate. Data were presented as mean ± SEM, with n = 3. Statistical significance was indicated as *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

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