Fig. 1: ALKBH4 expression is upregulated and correlated with poor prognosis in GC.
From: ALKBH4 impedes 5-FU Sensitivity through suppressing GSDME induced pyroptosis in gastric cancer
A The expression of ALKBH4 mRNA in different types of tumor tissues and normal tissues in TIMER database. *p < 0.05, **p < 0.001, ***p < 0.0001. B Pan-cancer analysis of ALKBH4 mRNA expression was performed using TCGA and GTEx data. C ROC curve was used to evaluate the accuracy of ALKBH4 in the diagnosis of gastric cancer. D The differential expression of ALKBH4 in cancer and adjacent tissues was analyzed based on TCGA-STAD data. The data were analyzed by paired t-test. E Based on RNA-Seq data, ALKBH4 in gastric adenocarcinoma was analyzed in detail in Normal and Tumor. F The bar chart represents the proportion of tumor samples showing higher expression of ALKBH4 compared with normal samples at each quantile cut-off value (minimum, 1st quartile, median, 3rd quartile, maximum). The specificity is calculated by dividing the number of tumor samples by the sum of tumor and normal samples below each given cut-off value. When the fold change exceeds 1, use “more than” instead of “less than”. G–I Kaplan–Meier plotter was used to obtain OS, PF, and PPS curves of GC patients with low and high expression of ALKBH4. J Representative immunohistochemical staining of ALKBH4 in GC and normal tissues. K IHC statistical analysis of TMA showed the proportion of ALKBH4 expression in GC tissues and normal tissues. L OS Kaplan–Meier survival curve based on ALKBH4 expression. M ΔIRS of ALKBH4 based on TMA. N Quantitative analysis of ALKBH4 levels in GC tissues and normal tissues. The data were analyzed by paired t-test. O The expression of ALKBH4 protein in the lysate of fresh gastric cancer and adjacent normal tissues. β-actin was used as an internal control. T tumor tissue, N normal tissue. P To compare the differential expression of ALKBH4 protein in gastric cancer cells and normal gastric mucosal cells. β-actin was used as an internal control. Data are expressed as means ± standard errors of the means of three independent experiments, *P < 0.05, **P < 0.01.
