Fig. 3: ALKBH4 negatively regulates the expression of GSDME in GC.
From: ALKBH4 impedes 5-FU Sensitivity through suppressing GSDME induced pyroptosis in gastric cancer
A, B The mRNA levels of GSDME, NLRP3, GSDMD, Caspase 1, and GSDMD were analyzed by qPCR after overexpression and knockdown of ALKBH4 in MKN45 and HGC-27 cells. C, D Western blot was used to detect the protein expression levels of GSDME, NLRP3, GSDMD, Caspase 1, and GSDMD after overexpression and knockdown of ALKBH4 in MKN45 and HGC-27 cells. E Immunofluorescence assay was employed to analyze the impact of ALKBH4 overexpression or ALKBH4 knockdown on the expression level of GSDME in gastric cancer cells MKN45 and HGC-27. Quantitative analysis of the fluorescence intensity showed on the right side. F Representative immunohistochemical staining of GSDME in GC and normal tissues. G Quantitative statistics on the expression of GSDME in 97 cases of gastric cancer tissues and 83 cases of normal gastric tissues. H OS Kaplan–Meier survival curve based on ALKBH4 expression. Data are expressed as means ± standard errors of the means of three independent experiments, *P < 0.05, **P < 0.01.
