Fig. 4: ALKBH4 mediates the transcriptional activation of GSDME by reducing the modification level of H3K4me3 in the promoter region of GSDME.
From: ALKBH4 impedes 5-FU Sensitivity through suppressing GSDME induced pyroptosis in gastric cancer
A, B GO biological process analysis and GSEA of differential genes involved in ALKBH4. C Enrichment of H3K4me3 binding peak in GSDME promoter region. D, E Protein expression levels of H3K4me3, H3K4me1, H3K9me3, H3K36me3, H3K56me3, and H3K64me3 were assessed using Western blot analysis following ALKBH4 overexpression and knockdown in GC cells. F The spatial distribution of 11 primer sets targeting the truncated fragments of the GSDME promoter was determined. G ChIP results of anti-IgG and anti-ALKBH4 antibodies in MKN45 cells. H, I To assess the binding efficiency of H3K4me3 to the GSDME promoter region, CHIP assay was performed following ALKBH4 overexpression and knockdown in MKN45 and HGC-27 cells. Data are expressed as means ± standard errors of the means of three independent experiments, *P < 0.05, **P < 0.01.
