Fig. 6: ALKBH4 reduced the chemosensitivity of gastric cancer cells to 5-FU through GSDME.

A The expression of IL-1β and IL-18 in GC cells overexpressing ALKBH4 after 5-FU treatment was analyzed by ELISA. B ELISA was used to analyze the expression of IL-1β and IL-18 in the supernatant of GC cells that underwent ALKBH4 knockdown. C We examined the effect of ALKBH4 overexpression on 5-FU-induced cytotoxicity and evaluated the effect of knockdown of ALKBH4 on the cytotoxicity of MKN45 and HGC-27 cells. D, E Chromatin integrity was detected by TUNEL. Quantitative analysis of the fluorescence intensity showed on the right side. F PI staining of MKN45 and HGC-27 cells was examined by fluorescence microscope. Quantitative analysis of the fluorescence intensity showed on the right side. G Flow cytometry was used to detect cell pyroptosis. H Western blot was used to detect the protein expression of GSDME and Cleaved-Caspase 3 after gastric cancer cells were treated with different concentrations of 5-FU. C-Caspase 3: Cleaved-Caspase 3. I The protein expression of GSDME and Cleaved-Caspase 3 was detected by western blot after 5-FU treatment of ALKBH4 knockdown gastric cancer cells. Data are expressed as means ± standard errors of the means of three independent experiments, *P < 0.05, **P < 0.01.