Fig. 1: The cellular composition and proportions in ACP as determined by snRNA-seq.

A Representative histology of peritumoural areas near (left; replete with glial tissue) and far from hypothalamus (right; adjacent to loose fibrous stroma with blood vessels) in ACP. Scale bar: 100 μm. B Scheme of the overall study procedure. The ACP tissues adjacent to the hypothalamus and paired ACP tissues away from the hypothalamus were collected for snRNA-seq and spatial transcriptomic analysis. C UMAP of 58,081 nuclei showing 10 clusters in different colours. D Dot plot showing marker genes for 10 clusters coloured by expression levels. The dot size represents the proportion of nuclei that expressed the genes in each cluster. E Stacked bar plots showing proportions and cell numbers of 10 clusters in each sample, and (F) pie chart indicating the overall percentage of each cluster in ACP.