Fig. 3: Characterization of myeloid cells in ACP. | Cell Death & Disease

Fig. 3: Characterization of myeloid cells in ACP.

From: Multiomics integration-based immunological characterizations of adamantinomatous craniopharyngioma in relation to keratinization

Fig. 3

A UMAP of myeloid cells, with 10 subclusters in different colours. B Stacked bar plot showing the proportion of 10 subclusters in each sample. C Violin plot showing the expression of marker genes. D Differences in specific function-related phenotype activities scored by GSVA among subclusters of myeloid cells except 3 DC subclusters. The scores are z score normalized. E Comparison of the TAM_GPNMB infiltration proportion between ACP samples and normal brain samples. F Representative mIHC staining of CD163 (red), RORA (green), LYVE1(blue) and GPNMB (yellow) in ACP. 3 myeloid subclusters are shown in individual channels respectively on the right. Scale bar (right): 100 μm (top, middle) and 200 μm (bottom). G RNA velocity plot of myeloid cells showing the dynamic shift in the cell state. H, I UMAP plots showing the distribution of Mo-like/MG-like scores. J Scatterplot of M1 and M2 signature scores for each nucleus among 4 TAM subclusters. K Heatmap showing the normalized TF activity of the top 10 regulons for each subcluster in myeloid cells. The dotted black box indicates the top 10 regulons in TAM_GPNMB, and the regulon of interest is marked in red. L UMAP plot showing the expression of ATF3. M Enriched signature of IRF8 and its downstream gene signature in ACP by GSEA. NES, normalized enrichment score.

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