Fig. 4: Characterization of epithelial cells in ACP. | Cell Death & Disease

Fig. 4: Characterization of epithelial cells in ACP.

From: Multiomics integration-based immunological characterizations of adamantinomatous craniopharyngioma in relation to keratinization

Fig. 4

A UMAP of epithelial cells clustered into 5 subpopulations in different colours. B Stacked bar plot showing the proportion of 5 subclusters in each sample. C Violin plot showing the expression of marker genes. D Violin plot showing palisade epithelium score among epithelial subclusters. E, F Comparison of the infiltration proportion for the E_C3 and E_C4 clusters between ACP samples and normal brain samples. G Bar plot of DEG enriched pathways for the E_C4 cluster by the Metascape tool. H The PPI network showing 7 complexes automatically identified by the Molecular Complex Detection (MCODE) algorithm in Metascape, coloured by each identity. Two major functional labels are listed on the right. I Differences in 8 epithelial expression program activities scored by GSVA among subclusters of epithelial cells, except the E_C5 cluster, the proliferative subcluster. J RNA velocity plot of epithelial cells showing cell transition directions. K IHC staining showing positivity of S100A4. Scale bar: 500 μm. mIHC showing the colocalization between S100A4 (red) and pan-keratin (green) in KCs. Scale bar: 50 μm. L Heatmap showing the normalized TF activity of the top 10 regulons for each subcluster in epithelial cells. The dotted black box indicates the top 10 regulons in E_C4, and the regulon of interest is marked in red. M, N UMAP plots showing the expression of ELF3 and EHF.

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