Fig. 6: Spatial colocalization and infiltration correlation of TAM_GPNMB and the E_C4 cluster.

A asmFISH of ACP tissues with keratins, showing the close spatial relationship between TAM_GPNMB and the E_C4 cluster. CD163 (green), GPNMB (red) and RHCG (white) are shown in individual channels respectively. H&E-stained adjacent section to show histology. The white arrows indicate TAM_GPNMB cells. The yellow arrows indicate E_C4 cells. Scale bar: 200 μm (left one, two), 200 μm (right one, two and three). B Pie charts showing the proportion of datasets with different correlation for infiltration of paired cell subtypes (Positive in red was defined as correlation coefficient [R] > 0.3; negative in blue was defined as R < −0.3; non-significant in grey). C The correlations for the infiltration of TAM_GPNMB and the E_C4 cluster in each dataset with ACP, including GSE68015 (n = 15; R = 0.57; p = 2.7e−02) and GSE94349 (n = 24; R = 0.66; p = 4.6e−04). The error band shows the 95% confidence interval. D The characteristics of cell infiltration in datasets. High infiltration is defined as the top twenty-five percent of infiltration group. E Enriched signature of epithelial mesenchymal transition, TNFα signalling via NF-κb and inflammatory response in group with high infiltration of TAM_GPNMB and E_C4 by GSEA.