Fig. 3: Expression analysis of mature neuronal markers in NEUROD1-hmOEG. | Cell Death & Disease

Fig. 3: Expression analysis of mature neuronal markers in NEUROD1-hmOEG.

From: Generation of functional neurons from adult human mucosal olfactory ensheathing glia by direct lineage conversion

Fig. 3

Representative images of neuronal markers SMI31 (A–D′) and synapsin (E–H′), expressed at 7, 14, 21, and 30 dpi. Yellow arrows indicate cells expressing the marker. Of note is the pattern of SMI31 expression in a positive gradient from the soma towards the axon ends (D’, white arrows), a characteristic axonal pattern. Synapsin labeling along the axon is also visible (H’, white arrowheads). GFP+ cells carry the NEUROD1-GFP construct. The histograms (I, J) represent the mean ± SD of the percentage of GFP+ cells expressing SMI31 or Synapsin over the total of GFP+ cells. Statistical tests applied were Two-way ANOVA and post-hoc Sidak test (****p ≤ 0.0001,*p ≤ 0.05) for multiple comparisons between means (n = 3 independent experiments, 25 fields were analyzed). Scale bar: 50 µm.

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