Fig. 5: KHSRP promotes pre-mRNA splicing through the interaction with SF3B1. | Cell Death & Disease

Fig. 5: KHSRP promotes pre-mRNA splicing through the interaction with SF3B1.

From: KHSRP ameliorates acute liver failure by regulating pre-mRNA splicing through its interaction with SF3B1

Fig. 5

A Spliced or unspliced mRNAs of KHSRP, SF3B1, EGFR, PHF5A, CDC25A, SSR4, and BCAP31 were measured using real-time PCR in stable KHSRP-knockdown HL7702 cells. B, C Spliced or unspliced EGFR, SF3B1, PHF5A, and CDC25A mRNAs were detected in (B) HL7702 cells with stable overexpression of KHSRP in the presence or absence of PB or (C) KHSRP-knockdown cells transfected with pHAGE-KHSRP-FL or pHAGE-KHSRP-ΔKH4Q. D Protein levels of KHSRP, EGFR, PHF5A, CDC25A, SF3B1, and SSR4 were detected in KHSRP-knockdown cells transfected with pHAGE-KHSRP-FL or pHAGE-KHSRP-ΔKH4Q using Western blotting. Data represent means ± SEM from three independent experiments. *p < 0.05; NS, no significant difference compared with the control.

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