Fig. 5: IL-27 ameliorates γδ T17 cells mediated psoriatic skin inflammation.
a Flow cytometry of IL-27Ra levels in γδ T cells isolated from the skin tissues of healthy or IMQ-induced psoriasis mice. b–f Cd2-Cre and Cd2-Cre Il27raflox/flox mice (n = 7) were treated with IMQ daily for 7 days. b Representative images of dander and H&E-stained sections were shown. Scale bar, 100μm. c Body weight change. d PASI score. e Spleen weight. f Flow cytometry analysis of IL-17 in skin γδ T cells. g–k In vitro cultured γδ T cells from Cd2-Cre (n = 4) or Cd2-Cre Il27raflox/flox (n = 6) mice that were differentiated under γδ T17 priming condition were transferred into TCRδ-KO mice (5 × 105 cells/mouse) two days before induction of psoriasis via IMQ. g Representative images of dander and H&E-stained sections were shown. Scale bar, 100 μm. h Body weight change. i PASI score. j, k Flow cytometry analyses of IL-17 in skin γδ T cells. l–o WT mice were intracutaneous administrated with rmIL-27 precautionarily (33.3 ng/kg/dose on Day −1, Day 1 and Day 3) or therapeutically (a single dose of injection on Day 3, 100 ng/kg) for the treatment of IMQ-induced psoriasis (n = 9 for PBS and n = 10 for the other groups). l Representative images of dander and H&E-stained sections were shown. Scale bar, 100 μm. m PASI score. n Spleen weight. o Flow cytometry analysis of IL-17 in skin γδ T cells. p–r Naïve or psoriatic mice were intracutaneous administrated with rmIL-27 on Day 3 post IMQ treatment (100 ng/kg), skin lymphocytes were isolated and dermal γδ T cells were analyzed (n = 7). Representative FACS plots and statistical analysis of mean fluorescent intensity for MitoTracker (p), JC-1 (q) and Bidipy (r). Data were presented as mean ± SEM, statistical differences were performed using two-tailed unpaired student’s t-test (a, e, f, j), One-way ANOVA (n–r) or Two-way ANOVA (c, d, h, i, m). *p < 0.05, **p < 0.01, ***p < 0.001, NS not significant.
