Fig. 3: UBTD1 promotes colorectal cancer cell proliferation and migration by regulating c-Myc protein.

A Gene set enrichment analysis based on the RNA-seq in UBTD1 overexpression HCT116 cells compared to the control HCT116 cells. B Volcano plot based on proteomics in UBTD1 overexpression HCT116 cells compared to control HCT116 cells. C The mRNA expression of c-Myc in UBTD1 overexpression or knockdown HCT116 and P53R cells. D The protein expression of c-Myc in UBTD1 overexpression or knockdown HCT116 and P53R cells. E Decreased colony formation caused by UBTD1 knockdown can be rescued by upregulation of c-Myc in HCT116 and P53R cells. F Decreased migration capability caused by UBTD1 knockdown can be rescued by upregulation of c-Myc in HCT116 and P53R cells. G Downregulation of c-Myc expression effectively reverses the augmented xenograft tumor proliferation facilitated by overexpression of UBTD1. *P < 0.05, **P < 0.01, or *** P < 0.001 indicates a significant difference between the indicated groups.