Fig. 4: UBTD1 promotes aerobic glycolysis in colorectal cancer cells by regulating the expression of hexokinase 2 via upregulating c-Myc. | Cell Death & Disease

Fig. 4: UBTD1 promotes aerobic glycolysis in colorectal cancer cells by regulating the expression of hexokinase 2 via upregulating c-Myc.

From: The ubiquitin-like protein UBTD1 promotes colorectal cancer progression by stabilizing c-Myc to upregulate glycolysis

Fig. 4

A Principal component analysis showed a distinctive principal component 1 pattern between the UBTD1 overexpression group and the control group. B Enhanced glycolysis was observed in UBTD1 overexpression HCT116 cells with elevated G6P, F6P, pyruvate, and lactate via metabonomics analysis. C The overexpression and knockdown of UBTD1 can respectively increase or decrease glucose uptake and lactate production in HCT116 and P53R cells. D Several key glycolysis-related genes were detected by WB in UBTD1 overexpression or knockdown HCT116 and P53R cells, showing HK2 was significantly associated with UBTD1 expression. E The construction of HK2 interfering plasmid, and validation in UBTD1 overexpressed or control HCT116 cells. F The promotion effect on lactate production and glucose uptake after UBTD1 overexpression could be diminished by HK2 knockdown in HCT116 cells. G HK2 was significantly upregulated after c-Myc overexpression both at mRNA and protein levels through qRT-PCR and WB assay in HCT116 and P53R cells. H Downregulation of HK2 caused by UBTD1 knockdown can be rescued by overexpression of c-Myc in HCT116 and P53R cells. *P < 0.05, **P < 0.01, or ***P < 0.001 indicates a significant difference between the indicated groups.

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