Fig. 1: PRMT1 responds to high glucose to promote NSCLC cell proliferation.
From: PRMT1 promotes Warburg effect by regulating the PKM2/PKM1 ratio in non-small cell lung cancer
A A549 cells were cultured in medium containing glucose at the indicated concentrations for 18 h and cell lysates subjected to immunoblot analysis with asymmetric arginine methylation (ASYM) or symmetric arginine methylation (SYM) antibodies. B A549 cells were cultured in medium containing glucose at the indicated concentrations for 18 h and lysates subjected to immunoblot analysis with PRMT1, PRMT4, or PRMT6 antibodies. Cyclin A was used as the control. C A549 cells were glucose-starved for 12 h, followed by stimulation with glucose (25 mM) for the indicated times, and lysates subjected to immunoblot analysis. D A549 cells were treated with melatonin at different concentrations for 24 h. E A549 cells overexpressing PRMT1 were stimulated with melatonin and proliferation rates measured via CCK-8 assays. F Proliferation of A549 cells overexpressing PRMT1 and treated with melatonin was evaluated using the monoclonal assay. Error bars, mean ± SD. ns (non-significant), P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.
