Fig. 3: PRMT1 mediates the splicing of PKM through modulation of PTBP1.
From: PRMT1 promotes Warburg effect by regulating the PKM2/PKM1 ratio in non-small cell lung cancer
A qPCR analysis of genes encoding glucose metabolism and OXPHOS-related enzymes in A549 cells transfected with the shCtrl and shPRMT1#1 constructs. B Western blot analysis of PKM1 and PKM2 in A549 and H1299 cells with PRMT1 knockdown. C, D Western blot and qPCR analyses of PTBP1, hnRNPA1, and hnRNPA2B1 in A549 and H1299 cells with PRMT1 knockdown. E, F Western blot and qPCR analyses of PKM1 and PKM2 in A549 cells transfected with the vector, shPRMT1#1, and shPRMT1#2+ Flag-PTBP1 constructs. G–I Western blot and qPCR analyses of PTBP1, PKM1, and PKM2 in A549-vector and A549-Flag-PRMT1 cells under the condition of glucose insufficiency. J PTBP1 promoter fragments of different sizes were constructed and the interactions between PRMT1 and PTBP1 promoter detected via the dual luciferase reporter gene assay. K A549 cells were treated with AMI-1 (50 μM). ChIP assays were performed using the anti-H4R3asym2 antibody and immunoprecipitated DNA analyzed via qPCR using PTBP1-specific primers. Error bars, mean ± SD. ns (non-significant), P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
