Fig. 5: Glucose inhibits ubiquitylation of PRMT1 through promoting USP7 expression in p53 wild-type NSCLC.
From: PRMT1 promotes Warburg effect by regulating the PKM2/PKM1 ratio in non-small cell lung cancer
A Mass spectrometry analysis of Flag-PRMT1 protein complex purified from A549-Flag-PRMT1 cells via anti-Flag immunoaffinity chromatography. B Western blot analysis of PRMT1 in A549-shCtrl, shUSP7#1, and shUSP7#2 cells. C Western blot analysis of PRMT1 in A549-shCtrl, shUSP9X#1, and shUSP9X#2 cells. D Interactions between PRMT1 and USP7 detected via co-immunoprecipitation (Co-IP) assay with anti-Flag or anti-His antibodies in HEK-293T cells transfected with the Flag-USP7 and His-PRMT1 constructs, respectively. E, F Western blot analysis of PRMT1 in A549-shCtrl, A549-shUSP7#1, and A549-shUSP7#2 cells treated with CHX (100 μg/mL) or MG132 (10 μM). G HEK-293T cells were transfected with USP7 WT or USP7 C223S and cell lysates immunoprecipitated using anti-His antibody, followed by immunoblotting analysis. H, I Western blot analysis of PRMT1 in A549-Flag-USP7 cells subjected to glucose deprivation and treated with melatonin. J, K Western blot analysis of PRMT1 ubiquitination after immunoprecipitation with PRMT1 antibody in A549-Vector and A549-Flag-USP7 cells subjected to glucose deprivation or melatonin treatment. L Western blot analysis of USP7 in A549 and H1299 cells with p53 overexpressed. M Expression of USP7 in A549 cells treated with pifithrin-α under glucose insufficiency. N Western blot analysis of PRMT1 protein expression in A549-Vector, A549-p53, and A549-p53+Flag-USP7 cells.
