Fig. 1: NOP2 is upregulated in lung cancer tissues and predicts poor prognosis.

A TIMER 2.0 database was used for pan‐cancer analysis of NOP2. B, C Differential analysis of NOP2 expression in LUAD and normal tissues. D, E Kaplan–Meier analysis and receiver operating characteristic curve of NOP2. F The heat map of the relationship between NOP2 expression and different clinicopathologic features of LUAD. G, H Forest plots of cox regression analysis of the relationship between NOP2 expression and different clinicopathologic features of LUAD. I Representative IHC staining images and J IHC scores of NOP2 expression in paired LUAD tissues and adjacent non-tumor tissues. Scale bar = 50 μm. K Western blot for NOP2 expression in several specimens from LUAD patients. L, M RT-qPCR and western blot were used to compare the difference in NOP2 expression in lung cancer cells and normal human lung epithelial cells. Statistical methods: independent samples t-test (J), one-way ANOVA (L). Data are presented as the mean ± SEM of at least 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.