Fig. 4: EZH2 is a downstream gene of NOP2 in lung cancer cells.

A, D Volcano plot and heat map of RNA-sequencing showing the genes whose expression level was positively or negatively correlated with the expression level of NOP2. B, C GO and KEGG analysis for differential genes obtained by RNA-sequencing. E RT-qPCR was performed to detect mRNA levels of five differential genes associated with migration and invasion. F STRING database analysis of the protein interaction relationship. The types of connection represented by the different colored edges between the nodes are indicated in the figure. EZH2 expression upon NOP2 knockdown or overexpression were determined by G, H RT-qPCR and I, J western blot. K ENCORI database analysis of the correlation between NOP2 and EZH2 expression in LUAD. L Differential analysis of EZH2 expression in LUAD and normal tissues. M Kaplan–Meier analysis of EZH2. N The heat map of the relationship between EZH2 expression and different clinicopathologic features of LUAD. Statistical methods: independent samples t-test (E, G), one-way ANOVA (H). Data are shown as the mean ± SEM of at least 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.