Fig. 7: EZH2 deficiency can counterbalance the positive effect of NOP2 on lung cancer cells in vitro. | Cell Death & Disease

Fig. 7: EZH2 deficiency can counterbalance the positive effect of NOP2 on lung cancer cells in vitro.

From: NOP2 facilitates EZH2-mediated epithelial–mesenchymal transition by enhancing EZH2 mRNA stability via m5C methylation in lung cancer progression

Fig. 7

A RT-qPCR was performed to detect the expression level of E-cadherin mRNA in each group of H1299 cells. B, C Binding of EZH2 and H3K27me3 antibodies to the E-cadherin promoter was detected by ChIP assay in H1299 cells. D–F Set up opposite NOP2 and EZH2 expression groups and do the same experiments in H1650 cells. Transwell (G–J) and wound-healing assays (K–N) were performed to detect the migratory and invasive abilities of lung cancer cells. Scale bar = 100 μm. Statistical methods: one-way ANOVA (A–F, H, J, L, N). Data are presented as the mean ± SEM of at least 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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