Fig. 4: Erianin promotes TMZ sensitivity in TMZ-resistant GSCs through ferroptosis. | Cell Death & Disease

Fig. 4: Erianin promotes TMZ sensitivity in TMZ-resistant GSCs through ferroptosis.

From: Erianin induces ferroptosis in GSCs via REST/LRSAM1 mediated SLC40A1 ubiquitination to overcome TMZ resistance

Fig. 4

a, b Cell viability of U87R and GSCm01R measured using CCK-8 after treatment with TMZ and erianin combined with Fer-1 at different times. c, d Representative EdU assay showing proliferation and quantitative analysis of U87R and GSCm01R after treatment with TMZ and erianin followed by Fer-1. Scale bar = 50 μm. e, g Migration assays of U87R and GSCm01R using a Transwell 24-well plate without extracellular matrix gel and quantitative analysis after treatment with TMZ and erianin combined with Fer-1. Scale bar = 50 μm. f, h Migration assays of U87R and GSCm01R using a Transwell 24-well plate with extracellular matrix gel and quantitative analysis after treatment with TMZ and erianin combined with Fer-1. Scale bar = 50 μm. i, j NSFA showing the size of neurospheres and quantitative analysis of GSCm01R after treatment with TMZ and erianin combined with Fer-1. Scale bar = 50 μm. k ELDA showing the neurosphere-forming ability of GSCm01R after treatment with TMZ and erianin combined with Fer-1. l Western blotting of stemness marker in GSCm01R after treatment with TMZ and erianin combined with Fer-1. All data are shown as the mean ± SD (five independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ns no significance.

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