Fig. 1: Expression of Shp2 in various retinal cells. | Cell Death & Disease

Fig. 1: Expression of Shp2 in various retinal cells.

From: Age-related retinal degeneration resulting from the deletion of Shp2 tyrosine phosphatase in photoreceptor neurons

Fig. 1

Isolated bovine rods were immunostained with Shp2 (green) and rhodopsin (red) antibodies (A–C). ROS, rod outer segments, RIS, rod inner segments, CC, connecting cilium. Retina lysate was subjected to Opti-Prep (8–40%) density gradient centrifugation and the fractions were immunoblotted with Shp2 and opsin (rhodopsin) (D). Cross section of the retina displaying all the retinal cells (E). Translating Ribosome Affinity Purification. Floxed Rpl22 mice were mated with rod-Cre (F), cone-Cre, Müller cell-Cre, RPE-Cre, and RGC-Cre individually and the ribosomes were immuno-affinity purified with HA antibody. The bound mRNA associated with ribosomes was isolated (F). Equal amounts of mRNA from various retinal cells were quantified using Shp2 and Gab1 primers, and the data was normalized to the housekeeping gene Rpl37 (G). Input represents the total retinal RNA. Data are mean ± SEM (n = 3). Panels (E, F) were created with BioRender.com. Note: The opsin blot shown in (D) was previously used in another study [41]. The same samples were used to examine Shp2 expression.

Back to article page