Fig. 1: Pex2 null allele affects villus length and ISC number in murine small intestines. | Cell Death & Disease

Fig. 1: Pex2 null allele affects villus length and ISC number in murine small intestines.

From: Peroxisomal cholesterol metabolism regulates yap-signaling, which maintains intestinal epithelial barrier function and is altered in Crohn’s disease

Fig. 1

A Representative image of dissected guts from WT and Pex2−/− pups at postnatal day 0. The bar graph on the right represents the quantification in cm of the length of the intestinal tract in WT and Pex2−/− pups at day 0. B Images of hematoxylin and eosin staining of small intestine sections from 3 WT and 3 Pex2−/− pups at day 0 pups; the small intestine was divided into three parts roughly corresponding to the duodenum, jejunum, and ileum. Scalebar = 50 µm. We measure the length and cell number/villus of at least 50 villi of 3–5 mice for each genotype. C Representative image of IF microscopy of the enterocyte marker sucrase-isomaltase in MIO. The bar graph on the right represents the quantification of the mean fluorescence intensity (MFI) per organoid region of interest (ROI); n = 30 organoids developed from three mice. Scalebar = 20 µm. D Representative imaging of the enteroendocrine cells marker chromogranin A in MIO. The bar graph on the right reports the quantification of the number of Chromogranin A-positive cells per MIO area; n = 30 organoids developed from three mice. Scalebar = 20 µm. E Imaging of Alcian Blue/PAS (goblet cells) staining of formalin-fixed, paraffin-embedded intestinal tissue sections. Red arrows indicate goblet cells. The histogram reports the number of Alcian Blue/PAS-positive cells per villus; n = 220 villi from four mice for each genotype. F Imaging of Olfm4, a marker for ISC. The histograms report the MFI value per crypt (top) and the number of Olfm4-positive cells per crypt (bottom); n = 30 crypts from three different mice per genotype. Scalebar = 10 µm. G The bar graphs report the relative expression of Lgr5 and Olfm4 transcripts in WT and Pex2−/− MIO at postnatal day 3 and day 15. Organoids were developed from n = 3 mice per genotype. H Imaging of pH3 in small intestine tissue cryosections. The bar graph represents the number of pH3-positive cells per crypt; n = 30 from three mice. scalebar = 50 µm. The error bars in all the graphs show the standard deviation, and significance was determined using Student’s t-test. ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant.

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