Fig. 2: Pex2−/− mice do not show increased cell death in the small intestine. | Cell Death & Disease

Fig. 2: Pex2−/− mice do not show increased cell death in the small intestine.

From: Peroxisomal cholesterol metabolism regulates yap-signaling, which maintains intestinal epithelial barrier function and is altered in Crohn’s disease

Fig. 2

A IF image of active caspase3 in Mio derived from WT and Pex2−/− small intestines. The bar graph represents the Mean Fluorescence Intensity (MFI) on the sum projection of fluorescence per region of interest (ROI). n = 30 organoids from three mice per genotype. Scalebar = 50 µm. B Image of TUNEL-positive cells in Mio derived from WT and Pex2−/− small intestines. The bar graph represents the arbitrary values of red positive-puncta per ROI. n = 30 organoids from three mice per genotype. Scalebar = 50 µm. C Detection of TUNEL-positive cells in MIO-derived monolayers. The bar graph represents the quantification of the number of TUNEL-positive dots in the total area reported in arbitrary units (A.U.). n = 30 monolayers derived from MIO from three mice per genotype. Scalebar = 200 µm. D Detection of TUNEL-positive cells in cryosections of the small intestine from WT and Pex2−/− pups. n = 30 total sections derived from three mice per genotype. Scalebar = 50 µm. In all the graphs, the error bars represent the standard deviation. Significance was determined using Student’s t-test. ns not significant.

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