Fig. 6: HIO-derived monolayers from IBD patients show peroxisome cholesterol-dependent recruitment of p-YAP out of the nuclei. | Cell Death & Disease

Fig. 6: HIO-derived monolayers from IBD patients show peroxisome cholesterol-dependent recruitment of p-YAP out of the nuclei.

From: Peroxisomal cholesterol metabolism regulates yap-signaling, which maintains intestinal epithelial barrier function and is altered in Crohn’s disease

Fig. 6

A Detection of peroxisomes SKL staining in HIO-derived monolayers for the detection of peroxisomes. For patient samples n = 10 monolayers. For healthy controls, n = 30. Scalebar = 10 µm. The bar graph reports the number of SKL-positive spots per cell in each sample. B Detection of p-YAP in HIO-derived monolayers. For patient samples, n = 10. For healthy controls, n = 30. Scalebar = 10 µm. The bar graph reports the percentage of the p-YAP fluorescent signal overlapping with the DAPI fluorescent signal. C p-YAP staining in HIO-derived monolayer from the patient with macroscopic Crohn’s disease, with and without treatment with 10 mM MβCD for 2 h. Scalebar = 10 µm. The bar graph reports the amount of p-YAP fluorescent signal that overlaps with the DAPI fluorescent signal. D The bar graph represents the quantification of fluorescence intensity of FITC-dextran in permeability assays of HIO-derived monolayers; n = 4 monolayers from four patients and four healthy control intestinal biopsies. In all histograms, the error bars represent standard deviations. Significance was determined using one-way ANOVA in A and B and Student’s t-test in C. ****p < 0.0001; **p < 0.01; *p < 0.05; ns not significant.

Back to article page