Fig. 2: GR/p63 interaction in keratinocytes.
a Venn diagram of ChIP-seq datasets for P63 bound to active enhancers (human) and GR (mouse). Note that almost 60% of GR bound genes are also bound by P63. b Functional categorization of overlapping GR and P63 bound genes in (a) for biological process with Metascape. c Venn diagram of up-regulated differentially expressed genes (DEG) in GR epidermal KO (GREKO) epidermis and p63-overexpressing epidermis. d Functional categorization of overlapping genes in (c) with Metascape, biological process. e p63 immunoblot of nuclear GR immunoprecipitations (IP) in mouse keratinocytes treated 1 h with vehicle (V), 100 nM dexamethasone (Dex), or 100 nM corticosterone (C). f Chromatin immunoprecipitation shows Dex-dependent modulation of GR and P63 recruitment to TP63 in N/TERT-2G (Dex, 1 μM, 1 h). g RT-QPCR for DNP63 evaluating response to Dex (1 μM, 3 h) in N/TERT-2G that were pre-incubated 16 h with V or 10μM RU486. Statistical significance using Student’s t-test (f, GR) or one-way ANOVA (f, P63; g) with post hoc Tukey multiple comparison test: *, #p < 0.05; **p < 0.01; ***p < 0.001. Asterisks: significant differences relative to vehicle (f, GR; g) or IgG (f, P63); hash: significant differences between groups indicated by brackets. N = 3 for all experiments.
