Fig. 4: IL4/IL13 treatment results in reduction of GR expression and function in human keratinocytes. | Cell Death & Disease

Fig. 4: IL4/IL13 treatment results in reduction of GR expression and function in human keratinocytes.

From: Glucocorticoid receptor controls atopic dermatitis inflammation via functional interactions with P63 and autocrine signaling in epidermal keratinocytes

Fig. 4

a Primary human keratinocytes were treated 72 h with vehicle or 30 ng/ml IL4/IL13 prior to immunoblotting (upper); quantitation (lower). N = 4. b Immunofluorescence of sections of human epidermal equivalents (HEEs) with primary keratinocytes treated with vehicle (V) or IL4/IL13 (30 ng/ml, 72 h) with indicated antibodies. c Graphs showing ratios of GR+ (top) or P63+ nuclei (bottom) relative to total DAPI stained nuclei in HEEs treated with vehicle (V) or IL4/IL13. Statistical significance determined using Student’s t-test, *p < 0.05; ***p < 0.001. N = 3. d Sections of HEEs with N/TERT-2G cells treated with vehicle (V) or IL4/IL13 (50 ng/ml, 72 h). Top panels: hematoxylin & eosin. Lower panels: immunofluorescence with indicated antibodies. Bars: 50 μm.

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