Fig. 5: Keratinocyte GR loss-of-function results in enhanced inflammation in human keratinocytes. | Cell Death & Disease

Fig. 5: Keratinocyte GR loss-of-function results in enhanced inflammation in human keratinocytes.

From: Glucocorticoid receptor controls atopic dermatitis inflammation via functional interactions with P63 and autocrine signaling in epidermal keratinocytes

Fig. 5

a Immunoblot (left) and quantitation (right) of control (CO) or GR-knockdown (GRKD) keratinocyte lysates with indicated antibodies. b, c CO or GRKD keratinocytes were treated 24 h with vehicle (V) or 50 ng/ml IL4/IL13. Immunoblotting (b) with P38 and phospho(p)-P38 antibodies (left); quantitation (right). RT-QPCR (c) for expression of indicated genes. Mean and SD are shown in graphs. Statistical significance using Student’s t-test (a) or 2-way ANOVA and post hoc Tukey multiple comparison test (b, c) indicated as: *, #p < 0.05; **, ##p < 0.01; ***, ###p < 0.001. Asterisks: statistically significant differences relative to CO V; hashes: comparisons between groups indicated by brackets. N = 3 for all experiments. d Heatmap shows relative levels of secreted factors in CO or GRKD primary keratinocyte supernatants (vehicle-treated) using a multiplex antibody array. Data are average of three independent experiments and represent factors with statistical significance using Student’s t-test; p < 0.05.

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