Fig. 6: Atopic dermatitis inflammation upon GR loss-of-function can be partially rescued by simultaneous GR/P63 knockdown. | Cell Death & Disease

Fig. 6: Atopic dermatitis inflammation upon GR loss-of-function can be partially rescued by simultaneous GR/P63 knockdown.

From: Glucocorticoid receptor controls atopic dermatitis inflammation via functional interactions with P63 and autocrine signaling in epidermal keratinocytes

Fig. 6

a Sections of GR-knockdown (GRKD) and control (CO) HEEs treated with vehicle (V) or 50 ng/ml IL4/IL13 (72 h) or 5μM RU486 (13 d) stained with hematoxylin & eosin (left panels and upper panel RU486) or P63-specific antibodies. Bars: 50 μm. N = 3. b Sections from normal (NS) or lesional (AD) skin. N = 2 for each condition. Bar: 300 μm. c, d CO or GRKD N/TERT-2G were transiently transfected with negative control or P63-specific (P63KD) siRNAs. Western blot (left) and quantification (right) show knockdown of GR and P63 (c). RT-QPCR for indicated genes (d). N = 3. Mean and SD are shown in graphs. Statistical significance using 2-way ANOVA (d) with post hoc Tukey multiple comparison test: *, #p < 0.05; **, ##p < 0.01; ***, ###p < 0.001. Asterisks: significant differences relative to CO; hashes: significant differences between groups indicated by brackets (d).

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