Fig. 2: NCAPH inhibits autophagy by regulating the formation of autophagosomes. | Cell Death & Disease

Fig. 2: NCAPH inhibits autophagy by regulating the formation of autophagosomes.

From: NCAPH, ubiquitinated by TRIM21, promotes cell proliferation by inhibiting autophagy of cervical cancer through AKT/mTOR dependent signaling

Fig. 2

A–D Acridine orange and LysoTracker staining showed no changes in the red fluorescence signals after silencing NCAPH, indicating that NCAPH has no significant effect on the pH of lysosomes. E–H Western blotting analysis showed that 3-MA (2 mM) treatment almost completely eliminated the changes in LC3B II caused by NCAPH interference. In contrast, bafilomycin A1(100 nM) treatment did not reverse the increase in LC3B II expression caused by interference with NCAPH in cervical cancer cells; I–K Western blotting analysis showed that silencing NCAPH dramatically increased the protein expression of Beclin-1, ATG 5 and ATG 7. The figure shows the representative results of three experiments. *P < 0.05, **p < 0.01, ***p < 0.001.

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