Fig. 1: Ubiquitylation at Lys360 promotes BCAT1 degradation through proteasomal pathway. | Cell Death & Disease

Fig. 1: Ubiquitylation at Lys360 promotes BCAT1 degradation through proteasomal pathway.

From: CHIP-mediated ubiquitin degradation of BCAT1 regulates glioma cell proliferation and temozolomide sensitivity

Fig. 1

A BCAT1 ubiquitination levels in HMC3, U251 and U87 cells were detected by immunoprecipitation and western blot. B HMC3, U251 and U87 cells were treated with 50 µg/mL cycloheximide, and BCAT1 expression was detected by western blot. C U251 cells were treated with 50 µg/mL cycloheximide with or without 10 µM MG132 or 10 µM chloroquine for 18 h, and BCAT1 expression was detected by western blot. D U251 cells were transfected with HA-tagged wild-type BCAT1 or BCAT1 mutant plasmid, and BCAT1 ubiquitination was detected by immunoprecipitation and western blot. E U251 cells were transfected with HA-tagged wild-type BCAT1 plasmid or BCAT1 mutant plasmid, and treated with 50 µg/mL cycloheximide. HA-BCAT1 expression was detected by western blot. Data were presented as mean ± SD of three independent experiments. ns p > 0.05, **p < 0.01, ***p < 0.001. CHX cycloheximide, CQ chloroquine.

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