Fig. 2: CHIP binds to BCAT1 through coiled-coil domain. | Cell Death & Disease

Fig. 2: CHIP binds to BCAT1 through coiled-coil domain.

From: CHIP-mediated ubiquitin degradation of BCAT1 regulates glioma cell proliferation and temozolomide sensitivity

Fig. 2

A Predicted ubiquitin ligase for BCAT1 on http://ubibrowser.bio-it.cn/ubibrowser/. B, C U251 cells were transfected with HA-BCAT1 with or without Flag-CHIP plasmid. The interaction between BCAT1 and CHIP was detected by immunoprecipitation and western blot. D Schematic diagram of wile-type CHIP and its truncated mutants. E U251 cells were transfected with HA-BCAT1 plasmid with or without Flag-tagged wild-type CHIP or CHIP mutant plasmid, and immunoprecipitation and western blot was performed. F U251 cells were transfected with Flag-tagged CHIP plasmid and HA-tagged wild-type BCAT1 or BCAT1 mutant plasmid, and immunoprecipitation and western blot was performed. G Subcellular location of BCAT1 and CHIP was detected by immunofluorescence. Scale bar = 50 μm. H In vitro binding assay of GST-BCAT1 and CHIP proteins.

Back to article page