Fig. 3: CHIP induces K48-linkage BCAT1 ubiquitylation at Lys360. | Cell Death & Disease

Fig. 3: CHIP induces K48-linkage BCAT1 ubiquitylation at Lys360.

From: CHIP-mediated ubiquitin degradation of BCAT1 regulates glioma cell proliferation and temozolomide sensitivity

Fig. 3

A U251 cells were co-transfected with HA-BCAT1 and His-ubiquitin plasmid with or without CHIP siRNA. BCAT1 ubiquitination was detected by immunoprecipitation and western blot. B U251 cells were co-transfected with HA-BCAT1 and His-ubiquitin plasmid with or without Flag-CHIP plasmid. Cells were then treated with 10 µM MG132 for 12 h, and BCAT1 ubiquitination was detected by immunoprecipitation and western blot. C U251 cells were co-transfected with HA-BCAT1 and His-ubiquitin plasmid with or without Flag-CHIP plasmid, and BCAT1 ubiquitination was detected by immunoprecipitation and western blot. D, E U251 cells were co-transfected with HA-BCAT1 and His-ubiquitin plasmid with or without Flag-CHIP plasmid. BCAT1 ubiquitin chain linkage was detected by immunoprecipitation and western blot. F U251 cells were co-transfected with HA-tagged wild-type BCAT1 plasmid or BCAT1 mutant plasmid and His-ubiquitin, Flag-CHIP plasmid. BCAT1 ubiquitination was detected by immunoprecipitation and western blot. G U251 cells were transfected with HA-BCAT1, His-ubiquitin plasmid and Flag-tagged wild-type CHIP plasmid or CHIP mutant plasmid. BCAT1 ubiquitination was detected by immunoprecipitation and western blot.

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