Fig. 4: CHIP promotes BCAT1 degradation. | Cell Death & Disease

Fig. 4: CHIP promotes BCAT1 degradation.

From: CHIP-mediated ubiquitin degradation of BCAT1 regulates glioma cell proliferation and temozolomide sensitivity

Fig. 4

A U251 cells were transfected with scramble siRNA or CHIP siRNA. BCAT1 and CHIP expression were detected by western blot. B U251 cells were transfected with Flag-tagged wild-type CHIP plasmid or CHIP mutant plasmid. BCAT1 and CHIP expression were detected by western blot. C U251 cells were transfected with scramble siRNA or CHIP siRNA, and treated with 50 µg/mL cycloheximide. BCAT1 expression were detected by western blot. D U251 cells were transfected with Flag-tagged wild-type CHIP plasmid or CHIP mutant plasmid, and treated with 50 µg/mL cycloheximide. BCAT1 expression were detected by western blot. E U251 cells were transfected with HA-BCAT-K360R plasmid with or without Flag-CHIP plasmid, and treated with 50 µg/mL cycloheximide. BCAT1 expression were detected by western blot. Data were presented as mean ± SD of three independent experiments. ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page