Fig. 6: CHIP-mediated BCAT1 degradation inhibits tumor growth in xenograft model. | Cell Death & Disease

Fig. 6: CHIP-mediated BCAT1 degradation inhibits tumor growth in xenograft model.

From: CHIP-mediated ubiquitin degradation of BCAT1 regulates glioma cell proliferation and temozolomide sensitivity

Fig. 6

A U87 cells stably expressing CHIP and BCAT1K360R were constructed. B–D Cell proliferation was detected by crystal violet staining and EdU staining. E–G Male NOD/SCID mice were subcutaneously injected with wild-type U87 cells, U87-CHIP cells or U87-CHIP/BCAT1K360R cells. Fourteen days later, mice were sacrificed and tumors were dissected and photographed (E). Volume (F) and weight (G) of xenograft tumors were measured. n = 5. H H&E staining and immunohistochemistry analysis of the sections of tumor. Scale bar = 50 μm. Data were presented as mean ± SD. ** p < 0.01, ***p < 0.001.

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