Fig. 2: PD-L1 promotes proliferation and motility of EGFR-mutant LUAD cells and inhibits cell apoptosis. | Cell Death & Disease

Fig. 2: PD-L1 promotes proliferation and motility of EGFR-mutant LUAD cells and inhibits cell apoptosis.

From: PD-L1 induces autophagy and primary resistance to EGFR–TKIs in EGFR-mutant lung adenocarcinoma via the MAPK signaling pathway

Fig. 2

A Western blotting verification of PD-L1 overexpression (PD-L1) in PC9 and HCC827 cells. B CCK-8 assay revealing the impacts of PD-L1 on PC9 and HCC827 cell growth. C. Colony formation assay showing the effects of PD-L1 on PC9 and HCC827 cell growth. D EDU assay revealing the effects of PD-L1 on PC9 and HCC827 cell proliferative abilities (scale bar, 150 μm). E Cell cycle assay revealing the differences of the proliferation index (sum of S and G2/M phases proportion) between the PD-L1 and vector groups of PC9 and HCC827 cell lines. F Cell apoptosis assay revealing the proportions of apoptotic PC9 and HCC827 cells in the PD-L1 and vector groups using Annexin V/7-AAD double staining. G The protein levels of PD-L1, cell cycle-related and apoptosis-related markers in PC9 and HCC827 cells were assessed by Western blotting. H Cell migration and invasion in the PD-L1 and vector groups of PC9 and HCC827 cells were measured by Transwell assay (scale bar, 150 μm; magnification, 20 ×). I. Wound-­healing assay comparing the cell migration differences among the PD-L1 and vector groups of PC9 and HCC827 cells (scale bar, 750 μm). The analyses were repeated three times. Data were presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Back to article page