Fig. 4: PD-L1 inhibits cell apoptosis by promoting autophagy via the MAPK signaling pathway in EGFR-mutant LUAD cells. | Cell Death & Disease

Fig. 4: PD-L1 inhibits cell apoptosis by promoting autophagy via the MAPK signaling pathway in EGFR-mutant LUAD cells.

From: PD-L1 induces autophagy and primary resistance to EGFR–TKIs in EGFR-mutant lung adenocarcinoma via the MAPK signaling pathway

Fig. 4

A The effects of PD-L1 overexpression on EGFR downstream signaling pathways activation were verified by Western blotting in PC9 and HCC827 cells. B The protein levels of p-ERK, ERK, apoptosis and autophagy-related proteins were assessed by Western blotting. PC9 PD-L1 and HCC827 PD-L1 cells were pretreated with MER/ERK pathway inhibitor PD98059. C PC9 and HCC827 cells as in (B) were immunostained with antibodies against LC3B (scale bar, 20 μm). D Apoptotic rate was measured using Annexin V/7-AAD staining in cells as in (B). E, G The viability of cells as in (B) was assessed by colony formation assay (E), EDU assay (F) and CCK-8 assay (G) (scale bar, 150 μm). H–K Images of tumors from nude mice (H), tumor weights (I), the tumor growth rates (J) and mice body weights (K) in vector, PD-L1 and PD-L1 + PD98059 groups. The analyses were repeated three times. Data were presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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