Fig. 3: The SYT13+/− transcriptome is characterized by impaired MN development and increased stress response. | Cell Death & Disease

Fig. 3: The SYT13+/− transcriptome is characterized by impaired MN development and increased stress response.

From: Heterozygous knockout of Synaptotagmin13 phenocopies ALS features and TP53 activation in human motor neurons

Fig. 3

A PCA displaying the transcriptional separation between SYT13+/− and SYT13+/+ MNs. B Heatmap showing the differentially expressed genes in SYT13+/− cultures. C Representative GO biological processes terms obtained by enrichment analysis based on the SYT13+/− transcriptome. D GSEA plots showing processes most related to SYT13+/+ or SYT13+/− neurons. E Confirmation of LHX3 and LHX4 expression in SYT13+/− MNs with rt-qPCR. N = 5 differentiations. F SYT13+/− MNs have fewer primary dendrites than isogenic controls. Scale bar 10 μm. N = 3 differentiations. G GSEA plot showing significant correlation between the SYT13+/− transcriptome and ALS ontology. H Western blot analysis showing reduced levels of NfH in SYT13+/− cultures. N = 3 differentiations. *p < 0.05. Data are represented as the mean ± SD.

Back to article page